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Q: How much does it cost to store plasmids in the repository? A: There is no cost to store plasmids in the repository, and no cost to your laboratory when other researchers order clones. In fact, one reason the plasmid repository was created was to alleviate the burden on individual labs to store, archive, and distribute the clones they create. Researchers who order clones are charged a reasonable fee (see prices). Q: What types of clones can be submitted to the repository? A: The current collection includes sequence verified cDNAs, shRNAs, and other plasmid clones. We're interested in housing any clones that might be of general use in the community, or any clones your lab receives requests for on a regular basis. We accept both small and large clone sets. Q: Why am I charged for the clones that I order? A: Storage of clones, storage of relevant information, quality control, data back-up and security, and plasmid clone distribution present significant costs to the DNA Resource Core in the form of equipment, supplies, and personnel. The charge for plasmid distribution partially offsets these charges. Funding from the DF/HCC and other sources helps us to keep prices low without compromising quality. Q: In what form should I submit clones to the repository? A: We prefer that you submit your clones as DNA, since we are concerned about T1 phage contamination, but there are other options as well. Once we receive the DNA, we transform it into an appropriate T1 Phage-resistant bacterial strain, establish archival and working glycerol stocks, and add them to the automated freezer storage system. Information about the plasmid clones will be curated and added to the database. If providing purified DNA rather than plasmid-containing bacteria places a significant burden on your resources or personnel, please contact Jason Kramer to discuss other options. Q: I didn't find a clone of interest in your collection. Are there other sources of Plasmid Clones? A: Yes, there are many other sources for plasmid clones, including individual labs, companies and non-profit sources. Please see our plasmid links page http://dnaseq.med.harvard.edu/plasmid-related_links.htm to get started finding other sources for clones.
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