Plasmid FAQs

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The DNA Resource Core plasmid repository offers:
  • Quality-controlled, automated storage of plasmid clones
  • Distribution of small or large sets of clones
  • Experiment-driven arraying of plasmid clones
  • Optional sequence verification of the clones you order

About the Plasmid Repository

The Plasmid Repository was created in response to a need for plasmid storage and distribution services created in part by the increase in genome-scale research efforts in DF/HCC member labs. 

Are you tired of shipping clones to colleagues? Concerned that precious resources and information files are being lost?  Wasting valuable research time managing plasmid clones? 

The DNA Resource Core offers:

  • Archival storage of glycerol stocks in our state-of-the-art, automated freezer system
  • Automated tracking of freeze/thaw cycles and storage time to ensure viability
  • Storage exclusively in T1 phage-resistant host strains
  • A storage capacity of over 80,000 individual samples (with plans in place for expansion)
  • Five years of experience storing and distributing tens of thousands of plasmid clones
  • Five years of experience providing high-quality, low cost services
  • Scientific curation of information that is then stored in an Oracle database
  • Scientific support for transfer of clones and information to our repository and database
  • Secure on-line ordering and regular back-up of information in the database

The repository itself is housed in a state-of-the-art, automated freezer storage system with an automatic picking station and 2D barcode reader, made possible through a grant from the National Institutes of Health. 

The collection already includes more than 30,000 clones, including sequence-verified cDNAs from humans and other organisms, expression vectors, and shRNAs.

For more information about the current plasmid clone collection, storing plasmid sets -- large or small -- or if you have other questions related to the plasmid repository and associated databases, please contact Jason Kramer.

 

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