Troubleshooting problems with DNA sequencing
Common causes of sequencing failures:
Too much or too little template DNA.
Click here to read our guidelines on DNA sample
preparation and DNA concentration.
Another common problem is high GC content.
Be sure to let us know if you expect your
DNA samples to have higher than average GC content. We will make
adjustments to the reaction mix (such as the addition of DMSO) to help overcome
problems created by high GC content.
Hairpins -- such as those in shRNA plasmid constructs -- can also be
problematic.
Our staff are expert at adjusting sequencing
conditions to sequence over hairpins and has experience sequencing shRNA
constructs. Please let us know if you are submitting sequences that you
know have hairpins or other potentially structure-forming sequences.
Click here to learn more about the Quality Report that is included with your sequence data.
Still having trouble? Please contact Li Chan at (617) 432-7708.
